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Eukaryotic Cell, February 2002, p. 66-74, Vol. 1, No. 1
1535-9778/02/$04.00+0     DOI: 10.1128/EC.01.1.66-74.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Saccharomyces cerevisiae C-Type Cyclin Ume3p/Srb11p Is Required for Efficient Induction and Execution of Meiotic Development

*** Katrina F. Cooper^, and Randy Strich^*

Program for Cell and Developmental Biology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111

Received 5 November 2001/ Accepted 9 November 2001

The yeast C-type cyclin Ume3p/Srb11p and its cyclin-dependent kinase partner Ume5p/Srb10p repress the transcription of several genes required for meiotic recombination or meiosis I nuclear division. To relieve this repression, Srb11p is destroyed early in meiosis, prior to the first meiotic division. This report identifies two roles for Srb11p in regulating meiotic development. First, SRB11 is required for the normal exit from the mitotic cell cycle prior to meiotic induction. Specifically, mutants lacking SRB11 (srb11) uncouple bud growth from chromosome segregation, producing small buds with nuclei. The bud growth defect is most likely due to the failure of srb11 mutants to reestablish polarized actin fibers at the bud tip following exposure to sporulation medium. Second, Srb11p is required for the efficient execution of meiosis I. srb11 mutants either exhibited a delay in performing meiosis I and meiosis II or skipped meiosis I entirely. This meiotic defect is not due to the activation of the recombination or spindle assembly checkpoint pathways. However, the expression of several meiotic genes is delayed and reduced in the mutant strains. These results suggest a positive role for Srb10-Srb11p in regulating the transcription program. This model is supported by the finding that overexpression of the meiotic inducer IME2 partially restored the ability of srb11 mutants to perform meiosis I. In conclusion, these findings indicate that Srb11p is required for both entry into and execution of the meiotic program, thus describing multiple roles for a C-type cyclin in the regulation of a developmental pathway.

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* Corresponding author. Mailing address: Program for Cell and Developmental Biology, Fox Chase Cancer Center, 7701 Burholme Ave., Philadelphia, PA 19111. Phone: (215) 728-5321. Fax: (215) 379-5715. E-mail: R___Strich{at}fccc.edu.

Present address: Department of Biochemistry, MCP-Hahnemann University, 245 N. 15th St., Philadelphia, PA 19102.

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Eukaryotic Cell, February 2002, p. 66-74, Vol. 1, No. 1
1535-9778/02/$04.00+0     DOI: 10.1128/EC.01.1.66-74.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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