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Eukaryotic Cell, December 2002, p. 906-914, Vol. 1, No. 6
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.6.906-914.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Loss of the F-Actin Binding and Vesicle-Associated Protein Comitin Leads to a Phagocytosis Defect

Thomas Schreiner,1 Martina R. Mohrs,1 Rosemarie Blau-Wasser,1 Alfred von Krempelhuber,2 Michael Steinert,3 Michael Schleicher,4 and Angelika A. Noegel1*

Center for Biochemistry, Medical Faculty, University of Cologne, 50931 Cologne,1 Max-Planck-Institut für Biochemie, 82152 Martinsried,2 Institut für Molekulare Infektionsbiologie, Universität Würzburg, 97070 Würzburg,3 Institut für Zellbiologie, Ludwig-Maximilians-Universität, 80336 Munich, Germany4

Received 11 November 2001/ Accepted 5 August 2002

Comitin is an F-actin binding and membrane-associated protein from Dictyostelium discoideum, which is present on Golgi and vesicle membranes and changes its localization in response to agents affecting the cytoskeleton. To investigate its in vivo functions we have generated knockout mutants by gene replacement. Based on comitin's in vitro functions we examined properties related to vesicular transport and microfilament function. Whereas cell growth, pinocytosis, secretion, chemotaxis, motility, and development were unaltered, comitin-lacking cells were impaired in the early steps of phagocytosis of Saccharomyces cerevisiae particles and of Escherichia coli, whereas uptake of latex beads was unaffected. Furthermore, the lack of comitin positively affected survival of pathogenic bacteria. Mutant cells also showed an altered response to hyperosmotic shock in comparison to the wild type. The redistribution of comitin during hyperosmotic shock in wild-type cells and its presence on early phagosomes suggest a direct involvement of comitin in these processes.

* Corresponding author. Mailing address: Institute of Biochemistry I, Joseph-Stelzmann-Str. 52, 50931 Cologne, Germany. Phone: 49-221-4786980. Fax: 49-221-4786979. E-mail: noegel{at}uni-koeln.de.

Eukaryotic Cell, December 2002, p. 906-914, Vol. 1, No. 6
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.6.906-914.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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Copyright © 2002 by the American Society for Microbiology.