Eukaryotic Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Lahondès, R.
Right arrow Articles by Arcangioli, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Lahondès, R.
Right arrow Articles by Arcangioli, B.

 Previous Article  |  Next Article 

Eukaryotic Cell, October 2003, p. 910-921, Vol. 2, No. 5
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.5.910-921.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Fission Yeast Sap1 Protein Is Essential for Chromosome Stability

Raynald de Lahondès,1 Veronique Ribes,2 and Benoit Arcangioli1*

Dynamique du Genome, URA 1644 du CNRS, Institut Pasteur, 75724 Paris, Cedex 15, France,1 Institute of Interdisciplinary Research (IRIBHN), School of Medicine, University of Brussels, B1070 Brussels, Belgium2

Received 31 March 2003/ Accepted 8 July 2003

Sap1 is a dimeric sequence-specific DNA binding-protein, initially identified for its role in mating-type switching of the fission yeast Schizosaccharomyces pombe. The protein is relatively abundant, around 10,000 dimers/cell, and is localized in the nucleus. sap1+ is essential for viability, and transient overexpression is accompanied by rapid cell death, without an apparent checkpoint response and independently of mating-type switching. Time lapse video microscopy of living cells revealed that the loss of viability is accompanied by abnormal mitosis and chromosome fragmentation. Overexpression of the C terminus of Sap1 induces minichromosome loss associated with the "cut" phenotype (uncoupling mitosis and cytokinesis). These phenotypes are favored when the C terminus of Sap1 is overexpressed during DNA replication. Fluorescence in situ hybridization experiments demonstrated that the cut phenotype is related to precocious centromere separation, a typical marker for loss of cohesion. We propose that Sap1 is an architectural chromatin-associated protein, required for chromosome organization.

* Corresponding author. Mailing address: Dynamique du Genome, URA 1644 du CNRS, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris, Cedex 15, France. Phone: 33 14568 8454. Fax: 33 14568 8906. E-mail: barcan{at}pasteur.fr.

Eukaryotic Cell, October 2003, p. 910-921, Vol. 2, No. 5
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.5.910-921.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Appl. Environ. Microbiol. Infect. Immun. J. Bacteriol.
Mol. Cell Biol. Microbiol. Mol. Biol. Rev. ALL ASM JOURNALS
Copyright © 2003 by the American Society for Microbiology.