Eukaryotic Cell
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Eukaryotic Cell, December 2005, p. 1998-2007, Vol. 4, No. 12
1535-9778/05/$08.00+0     doi:10.1128/EC.4.12.1998-2007.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Envoy, a PAS/LOV Domain Protein of Hypocrea jecorina (Anamorph Trichoderma reesei), Modulates Cellulase Gene Transcription in Response to Light

Monika Schmoll,1 Lisa Franchi,2 and Christian P. Kubicek1*

Research Division for Gene Technology and Applied Biochemistry, Institute for Chemical Engineering, Vienna University of Technology, Getreidemarkt 9/1665, A-1060 Vienna, Austria,1 Dipartimento di Biotecnologie Cellulari ed Ematologia, Sezione di Genetica Moleculare, Universita' di Roma "La Sapienzia," Policlinico Umberto I, Viale Regina Elena 32, 00161 Rome, Italy2

Received 23 July 2005/ Accepted 20 September 2005

Envoy, a PAS/LOV domain protein with similarity to the Neurospora light regulator Vivid, which has been cloned due to its lack of expression in a cellulase-negative mutant, links cellulase induction by cellulose to light signaling in Hypocrea jecorina. Despite their similarity, env1 could not compensate for the lack of vvd function. Besides the effect of light on sporulation, we observed a reduced growth rate in constant light. An env1PAS– mutant of H. jecorina grows significantly slower in the presence of light but remains unaffected in darkness compared to the wild-type strain QM9414. env1 rapidly responds to a light pulse, with this response being different upon growth on glucose or glycerol, and it encodes a regulator essential for H. jecorina light tolerance. The induction of cellulase transcription in H. jecorina by cellulose is enhanced by light in the wild-type strain QM9414 compared to that in constant darkness, whereas a delayed induction in light and only a transient up-regulation in constant darkness of cbh1 was observed in the env1PAS– mutant. However, light does not lead to cellulase expression in the absence of an inducer. We conclude that Envoy connects the light response to carbon source signaling and thus that light must be considered an additional external factor influencing gene expression analysis in this fungus.

* Corresponding author. Mailing address: Research Division for Gene Technology and Applied Biochemistry, Institute for Chemical Engineering, Vienna University of Technology, Getreidemarkt 9/1665, A-1060 Vienna, Austria. Phone: (43) 1 58801 17250. Fax: (43) 1 58801 17299. E-mail: ckubicek{at}mail.zserv.tuwien.ac.at.

Eukaryotic Cell, December 2005, p. 1998-2007, Vol. 4, No. 12
1535-9778/05/$08.00+0     doi:10.1128/EC.4.12.1998-2007.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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