Eukaryotic Cell
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Eukaryotic Cell, January 2008, p. 172-176, Vol. 7, No. 1
1535-9778/08/$08.00+0     doi:10.1128/EC.00055-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A New Assay for Promoter Analysis in Chlamydomonas Reveals Roles for Heat Shock Elements and the TATA Box in HSP70A Promoter-Mediated Activation of Transgene Expression{triangledown} ,{dagger}

Mukesh Lodha,{ddagger} Miriam Schulz-Raffelt,{ddagger} and Michael Schroda*

Institute of Biology II, Plant Biochemistry, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany

Received 23 February 2007/ Accepted 25 October 2007

The aim of this work was to identify cis-regulatory sequences within the Chlamydomonas HSP70A promoter that mediate its stimulatory effect on the expression of downstream promoters. For this, we deleted/mutated the HSP70A promoter and, using a new assay, quantified its stimulatory effect. Our results indicate that the effect is mediated largely by heat shock elements and the TATA box.

* Corresponding author. Mailing address: Institute of Biology II, Plant Biochemistry, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany. Phone: 49 761/203 2708. Fax: 49 761/203 2601. E-mail: michael.schroda{at}biologie.uni-freiburg.de

{triangledown} Published ahead of print on 9 November 2007.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

{ddagger} These authors contributed equally to this work.

Eukaryotic Cell, January 2008, p. 172-176, Vol. 7, No. 1
1535-9778/08/$08.00+0     doi:10.1128/EC.00055-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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