Eukaryotic Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Other Versions of this Article:
EC.00215-07v1
7/1/86    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barth, S.
Right arrow Articles by Michaeli, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barth, S.
Right arrow Articles by Michaeli, S.

 Previous Article  |  Next Article 

Eukaryotic Cell, January 2008, p. 86-101, Vol. 7, No. 1
1535-9778/08/$08.00+0     doi:10.1128/EC.00215-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Elucidating the Role of C/D snoRNA in rRNA Processing and Modification in Trypanosoma brucei{triangledown} ,{dagger}

Sarit Barth, Boaz Shalem, Avraham Hury, Itai Dov Tkacz, Xue-hai Liang, Shai Uliel, Inna Myslyuk, Tirza Doniger, Mali Salmon-Divon, Ron Unger, and Shulamit Michaeli*

The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel

Received 21 June 2007/ Accepted 28 September 2007

Most eukaryotic C/D small nucleolar RNAs (snoRNAs) guide 2'-O methylation (Nm) on rRNA and are also involved in rRNA processing. The four core proteins that bind C/D snoRNA in Trypanosoma brucei are fibrillarin (NOP1), NOP56, NOP58, and SNU13. Silencing of NOP1 by RNA interference identified rRNA-processing and modification defects that caused lethality. Systematic mapping of 2'-O-methyls on rRNA revealed the existence of hypermethylation at certain positions of the rRNA in the bloodstream form of the parasites, suggesting that this modification may assist the parasites in coping with the major temperature changes during cycling between their insect and mammalian hosts. The rRNA-processing defects of NOP1-depleted cells suggest the involvement of C/D snoRNA in trypanosome-specific rRNA-processing events to generate the small rRNA fragments. MRP RNA, which is involved in rRNA processing, was identified in this study in one of the snoRNA gene clusters, suggesting that trypanosomes utilize a combination of unique C/D snoRNAs and conserved snoRNAs for rRNA processing.

* Corresponding author. Mailing address: The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel. Phone: 972-3-5318068. Fax: 972-3-5318124. E-mail: michaes{at}mail.biu.ac.il

{triangledown} Published ahead of print on 2 November 2007.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, January 2008, p. 86-101, Vol. 7, No. 1
1535-9778/08/$08.00+0     doi:10.1128/EC.00215-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Appl. Environ. Microbiol. Infect. Immun. J. Bacteriol.
Mol. Cell Biol. Microbiol. Mol. Biol. Rev. ALL ASM JOURNALS
Copyright © 2008 by the American Society for Microbiology.