Eukaryotic Cell
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Eukaryotic Cell, April 2008, p. 664-674, Vol. 7, No. 4
1535-9778/08/$08.00+0     doi:10.1128/EC.00331-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Microneme Rhomboid Protease TgROM1 Is Required for Efficient Intracellular Growth of Toxoplasma gondii{triangledown} ,{dagger}

Fabien Brossier, G. Lucas Starnes, Wandy L. Beatty, and L. David Sibley*

Department of Molecular Microbiology, Washington University School of Medicine, 660. S. Euclid Avenue, St. Louis, Missouri 63110-1093

Received 4 September 2007/ Accepted 28 January 2008

Rhomboids are serine proteases that cleave their substrates within the transmembrane domain. Toxoplasma gondii contains six rhomboids that are expressed in different life cycle stages and localized to different cellular compartments. Toxoplasma rhomboid protein 1 (TgROM1) has previously been shown to be active in vitro, and the orthologue in Plasmodium falciparum processes the essential microneme protein AMA1 in a heterologous system. We investigated the role of TgROM1 to determine its role during in vitro growth of T. gondii. TgROM1 was localized in the secretory pathway of the parasite, including the Golgi apparatus and micronemes, which contain adhesive proteins involved in invasion of host cells. However, unlike other micronemal proteins, TgROM1 was not released onto the parasite surface during cell invasion, suggesting it does not play a critical role in cell invasion. Suppression of TgROM1 using the tetracycline-regulatable system revealed that ROM1-deficient parasites were outcompeted by wild-type T. gondii. ROM1-deficient parasites showed only modest decrease in invasion but replicated more slowly than wild-type cells. Collectively, these results indicate that ROM1 is required for efficient intracellular growth by T. gondii.

* Corresponding author. Mailing address: Department of Molecular Microbiology, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, MO 63110-1093. Phone: (314) 362-8873. Fax: (314) 362-3203. E-mail: sibley{at}borcim.wustl.edu

{triangledown} Published ahead of print on 29 February 2008.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, April 2008, p. 664-674, Vol. 7, No. 4
1535-9778/08/$08.00+0     doi:10.1128/EC.00331-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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