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Eukaryotic Cell, July 2008, p. 1222-1226, Vol. 7, No. 7
1535-9778/08/$08.00+0     doi:10.1128/EC.00007-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evidence of a Continuous Endoplasmic Reticulum in the Protozoan Parasite Entamoeba histolytica{triangledown} ,{dagger}

Jose E. Teixeira1 and Christopher D. Huston1,2*

Departments of Medicine,1 Microbiology and Molecular Genetics, University of Vermont College of Medicine, Burlington, Vermont 054052

Received 4 January 2008/ Accepted 7 February 2008

Entamoeba histolytica, the cause of amebiasis, is believed to have no continuous endoplasmic reticulum (ER), with ER functions occurring in vesicles. Here, using an ER-targeted green fluorescent protein fusion protein and fluorescence loss in photobleaching, we have unambiguously demonstrated the presence of a continuous ER compartment in living E. histolytica trophozoites.

* Corresponding author. Mailing address: Departments of Medicine and Microbiology and Molecular Genetics, Rm 320 Stafford Hall, University of Vermont, 95 Carrigan Dr., Burlington, VT 05405. Phone: (802) 656-9115. Fax: (802) 656-5322. E-mail: christopher.huston{at}uvm.edu

{triangledown} Published ahead of print on 15 February 2008.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, July 2008, p. 1222-1226, Vol. 7, No. 7
1535-9778/08/$08.00+0     doi:10.1128/EC.00007-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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