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Eukaryotic Cell, November 2009, p. 1739-1749, Vol. 8, No. 11
1535-9778/09/$08.00+0     doi:10.1128/EC.00115-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Complex Nature of the Genome in a Wine Spoilage Yeast, Dekkera bruxellensis{triangledown} ,{dagger}

Linda Hellborg* and Jure Piskur*

Department of Cell and Organism Biology, Lund University, Lund SE-223 62, Sweden

Received 16 April 2009/ Accepted 24 August 2009

When the genome organizations of 30 native isolates belonging to a wine spoilage yeast, Dekkera (Brettanomyces) bruxellensis, a distant relative of Saccharomyces cerevisiae, were examined, the numbers of chromosomes varied drastically, from 4 to at least 9. When single gene probes were used in Southern analysis, the corresponding genes usually mapped to at least two chromosomal bands, excluding a simple haploid organization of the genome. When different loci were sequenced, in most cases, several different haplotypes were obtained for each single isolate, and they belonged to two subtypes. Phylogenetic reconstruction using haplotypes revealed that the sequences from different isolates belonging to one subtype were more similar to each other than to the sequences belonging to the other subtype within the isolate. Reanalysis of the genome sequence also confirmed that partially sequenced strain Y879 is not a simple haploid and that its genome contains approximately 1% polymorphic sites. The present situation could be explained by (i) a hybridization event where two similar but different genomes have recently fused together or (ii) an event where the diploid progenitor of all analyzed strains lost a regular sexual cycle, and the genome started to accumulate mutations.

* Mailing address: Department of Cell and Organism Biology, Lund University, Sölvegatan 35, Lund SE-223 62, Sweden. Phone for Linda Hellborg: 46 46 222 7253. Fax: 46 46 222 4113. E-mail: Linda.Hellborg{at}cob.lu.se. Phone for Jure Piskur: 46 46 222 8373. Fax: 46 46 211 1982. E-mail: Jure.Piskur{at}cob.lu.se

{triangledown} Published ahead of print on 28 August 2009.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, November 2009, p. 1739-1749, Vol. 8, No. 11
1535-9778/09/$08.00+0     doi:10.1128/EC.00115-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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