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Eukaryotic Cell, November 2009, p. 1803-1807, Vol. 8, No. 11
1535-9778/09/$08.00+0     doi:10.1128/EC.00240-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Immortalized Human Brain Endothelial Cell Line HCMEC/D3 as a Model of the Blood-Brain Barrier Facilitates In Vitro Studies of Central Nervous System Infection by Cryptococcus neoformans{triangledown}

Kiem Vu,1 Babette Weksler,2 Ignacio Romero,3 Pierre-Olivier Couraud,4 and Angie Gelli1*

Department of Pharmacology, School of Medicine, University of California, Genome and Biomedical Sciences Facility, Davis, California,1 Division of Hematology-Medical Oncology, Weill Medical College of Cornell University, New York, New York,2 Department of Biological Sciences, The Open University, Walton Hall, Milton Keynes, United Kingdom,3 Institut Cochin, Centre National de la Recherche Scientifique UMR 8104, Institut National de la Santé et de la Recherche Médicale (INSERM) U567, Université René Descartes, Paris, France4

Received 20 August 2009/ Accepted 4 September 2009

Cryptococcus neoformans cells must cross the blood-brain barrier prior to invading the central nervous system. Here we demonstrate that the immortalized human brain endothelial cell line HCMEC/D3 is a useful alternative to primary brain endothelial cells as a model of the blood-brain barrier for studies of central nervous system infection.

* Corresponding author. Mailing address: Department of Pharmacology, School of Medicine, University of California, Genome and Biomedical Sciences Facility, Davis, CA. Phone: (530) 754-6446. Fax: (503) 752-7710. E-mail: acgelli{at}ucdavis.edu

{triangledown} Published ahead of print on 18 September 2009.

Eukaryotic Cell, November 2009, p. 1803-1807, Vol. 8, No. 11
1535-9778/09/$08.00+0     doi:10.1128/EC.00240-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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