Eukaryotic Cell
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EC Accepts, published online ahead of print on 15 August 2008
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Eukaryotic Cell doi:10.1128/EC.00418-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The transcriptome of photo-biological hydrogen production induced by sulphur deprivation in the green alga Chlamydomonas reinhardtii

Anh Vu Nguyen, Skye R. Thomas-Hall, Alizée Malnoë, Matthew Timmins, Jan H. Mussgnug, Jens Rupprecht, Olaf Kruse, Ben Hankamer, and Peer M. Schenk*

School of Integrative Biology, and Institute for Molecular Bioscience, University of Queensland, St. Lucia, Queensland 4072, Australia; Department of Biology/AlgaeBioTech Group, University of Bielefeld, Bielefeld, Germany

* To whom correspondence should be addressed. Email: p.schenk{at}uq.edu.au.


  Abstract

Photo-biological hydrogen production using microalgae is being developed into a promising clean fuel stream for the future. In this study, microarray analyses were used to obtain global expression profiles of mRNA abundance in the green alga Chlamydomonas reinhardtii at different time points before the onset and during the course of sulphur-depleted hydrogen production. These studies were followed by real-time quantitative RT-PCR and protein analyses. The present work provides new insights into photosynthesis, sulphur acquisition strategies and carbon metabolism-related gene expression during sulphur-induced hydrogen production. A general trend towards repression of transcripts encoding photosynthetic genes was observed. In contrast to all other LHCBM genes, the abundance of the LHCBM9 transcript (encoding a major light harvesting polypeptide) and its protein was strongly elevated throughout the experiment. This suggests a major remodelling of the photosystem II light harvesting complex as well as an important function of LHCBM9 under sulphur starvation and photo-biological hydrogen production. This paper presents the first global transcriptional analysis of C. reinhardtii before, during and after photo-biological hydrogen production under sulphur deprivation.






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