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Eukaryotic Cell, February 2002, p. 105-111, Vol. 1, No. 1
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.1.105-111.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Pumilio Homologue from Saprolegnia parasitica Specifically Expressed in Undifferentiated Spore Cysts

*** M. Gunnar Andersson and Lage Cerenius*

Department of Comparative Physiology, Evolutionary Biology Centre, Uppsala University, SE-752 36 Uppsala, Sweden

Received 26 June 2001/ Accepted 15 October 2001

The expression of spore-specific marker transcripts at different stages of the asexual life cycle of Saprolegnia parasitica was analyzed. One of the markers, designated puf1, was found to be expressed transiently upon each of several cycles of zoospore encystment and reemergence. The transcript is induced immediately upon zoospore encystment and is rapidly lost when a cyst is triggered to germinate. In nongerminating cysts, puf1 is maintained until a time point when the cysts can no longer be triggered to germinate and thus have become determined for zoospore reemergence. The results show that the cyst stage has two phases, of about equal duration, which are physiologically and transcriptionally distinct and that the transcriptional machinery of oomycetes is also active in nongerminating spores. puf1 encodes a putative mRNA binding protein belonging to a conserved class of proteins including the Drosophila melanogaster Pumilio protein, Caenorhabditis elegans FBF, and Saccharomyces cerevisiae Puf5, all of which are involved in regulation of gene expression by posttranscriptional mechanisms.

* Corresponding author. Mailing address: Dept. of Comparative Physiology, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18A, SE-752 36 Uppsala, Sweden. Phone: 46-18-471 28 04. Fax: 46-18-471 64 25. E-mail: Lage.Cerenius{at}ebc.uu.se.

Eukaryotic Cell, February 2002, p. 105-111, Vol. 1, No. 1
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.1.105-111.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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