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Eukaryotic Cell, October 2002, p. 774-786, Vol. 1, No. 5
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.5.774-786.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Eng1p, an Endo-1,3-ß-Glucanase Localized at the Daughter Side of the Septum, Is Involved in Cell Separation in Saccharomyces cerevisiae

Victoriano Baladrón,{dagger} Sandra Ufano,{dagger} Encarnación Dueñas, Ana Belén Martín-Cuadrado, Francisco del Rey, and Carlos R. Vázquez de Aldana*

Instituto de Microbiología-Bioquímica, Departamento de Microbiología y Genética, CSIC/Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain

Received 8 April 2002/ Accepted 2 July 2002

ENG1 (YNR067c), a gene encoding a new endo-1,3-ß-glucanase, was cloned by screening a genomic library with a DNA probe obtained by PCR with synthetic oligonucleotides designed according to conserved regions found between yeast exo-1,3-ß-glucanases (Exg1p, Exg2p, and Ssg1p). Eng1p shows strong sequence similarity to the product of the Saccharomyces cerevisiae ACF2 gene, involved in actin assembly "in vitro," and to proteins present in other yeast and fungal species. It is also related to plant glucan-binding elicitor proteins, which trigger the onset of a defense response upon fungal infection. Eng1p and Acf2p/Eng2p are glucan-hydrolyzing proteins that specifically act on 1,3-ß linkages, with an endolytic mode of action. Eng1p is an extracellular, heavily glycosylated protein, while Acf2p/Eng2p is an intracellular protein with no carbohydrate linked by N-glycosidic bonds. ENG1 transcription fluctuates periodically during the cell cycle; maximal accumulation occurs during the M/G1 transition and is dependent on the transcription factor Ace2p. Interestingly, eng1 deletion mutants show defects in cell separation, and Eng1p localizes asymmetrically to the daughter side of the septum, suggesting that this protein is involved, together with chitinase, in the dissolution of the mother-daughter septum.

* Corresponding author. Mailing address: Instituto de Microbiología-Bioquímica, Departamento de Microbiología y Genética, CSIC/Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain. Phone: (34) 923 294 675. Fax: (34) 923 224 876. E-mail: cvazquez{at}usal.es.

{dagger} Present address: Área de Bioquímica y Biología Molecular, Dpto. Química Inorgánica, Orgánica y Bioquímica, Facultad de Medicina, Universidad de Castilla-La Mancha/CRIB, 2001 Albacete, Spain.

{ddagger} Present address: Centro Nacional de Biotecnología, CSIC/UAM, Cantoblanco, 28049 Madrid, Spain.

Eukaryotic Cell, October 2002, p. 774-786, Vol. 1, No. 5
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.5.774-786.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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Copyright © 2002 by the American Society for Microbiology.