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Eukaryotic Cell, April 2004, p. 518-526, Vol. 3, No. 2
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.2.518-526.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Mitochondrial Histone-Like DNA-Binding Proteins Are Essential for Normal Cell Growth and Mitochondrial Function in Crithidia fasciculata

Nuraly K. Avliyakulov,1,2 Julius Lukes,3 and Dan S. Ray1,2*

Molecular Biology Institute,1 Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California,2 Institute of Parasitology, Czech Academy of Sciences and Faculty of Biology, University of South Bohemia, 37005 Ceské Budejovice, Czech Republic3

Received 26 September 2003/ Accepted 8 January 2004

The Crithidia fasciculata KAP2 and KAP3 proteins are closely related kinetoplast-specific histone-like DNA-binding proteins. The KAP2 and KAP3 genes are 46% identical and are arranged in tandem on the chromosomal DNA. Disruption of both alleles of either gene alone shows no detectable phenotype. However, replacement of both copies of the sequence encoding the entire KAP2 and KAP3 locus increases maxicircle mRNA levels two- to fourfold. These double-knockout cells are viable but grow extremely slowly, have reduced respiration and very abnormal cell morphologies, and accumulate numerous large vacuoles. The extreme phenotype of these mutant cells suggests an important role for the KAP2 and KAP3 proteins in mitochondrial metabolism and cell growth.

* Corresponding author. Mailing address: Molecular Biology Institute and Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, 405 Hilgard Ave., Los Angeles, CA 90095-1570. Phone: (310) 825-4178. Fax: (310) 206-7286. E-mail: danray{at}ucla.edu.

Eukaryotic Cell, April 2004, p. 518-526, Vol. 3, No. 2
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.2.518-526.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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